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Although it still eludes us what ratio of these regulators influence decisions at a particular time and place, our data show that one dose of wild-type gld-2 and gld-1 genes [ gld-2( +/ ) gld-1( +/ ) ] are competent for meiotic entry, but are insufficient for the terminal differentiation of spermatocytes in the absence of PUF-8, resulting in dedifferentiation-mediated germline tumors (Figure 7)

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JAMA , 333 (8), 652655.

If flushing is bothersome, try injecting at a slightly slower rate or using a colder injection site