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What does reconstitution look like in practice

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The apparent Michaelis-Mentes constant (K m ), maximum velocity for the enzyme preparation (V max ) and specific activity of the enzyme fraction obtained from the plot were 6.83 M, 0.46 nmoles ml reaction mixture-1 min -1 and 0.34 nmoles g protein -1 min -1

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115 MariM.CaballeroF.ColellA.MoralesA.CaballeriaJ.FernandezA.et al (2006)