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0.2 mol/L pH 7.8 phosphate buffer and the proxy matrix containing 0.9% NaCl in 10 mmol/L phosphate buffer, pH 7.8 were prepared once a week and kept at 4 C

Then, the mice were sacrificed on the eleventh day

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Once removed from the fridge, the pen can be stored at room temperature below 30C for up to 21 days