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2.6 Alizarin red staining (ARS) Following 14 days of osteogenic differentiation, cells were fixed with 4% paraformaldehyde for 30 min at room temperature, washed thrice with PBS, and subsequently stained with ARS staining solution (OriCell, Guangzhou, China) for 510 min

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Fractures were identified by ICD-10 codes ( Supplemental Table S3 )