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doi: 10.1196/annals.1338.067 44 VincentAMPerroneLSullivanKABackusCSastryAMLastoskieCet al
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Synthesis of glutamine in nucleic acids, lipids, and proteins 1
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Cell culture and treatments The murine macrophage cell line RAW 264.7 (TIB-71, American Type Culture Collection, Manassas, VA, USA) was cultured at 37C and under 5% CO 2 condition in Dulbeccos modified Eagles medium (DMEM) containing 4.5 g/L glucose, 10% fetal bovine serum (FBS), and 1% penicillinstreptomycin (P/S) mixture
1 H NMR spectroscopy analysis showed a large shift of two protons, H1 and H2 in Cys solution containing Hg (Supplementary Figure 2)
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