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MCF 10A (breast epithelial, ATCC CRL-10317) were cultured in high glucose Dulbeccos modified Eagles (DMEM) medium and HAMS F12 (1:1 ratio, Euroclone), supplemented with 5% horse serum (Gibco), 1% L-glutamine (Gibco), 1% penicillin/streptomycin (Gibco), 10 g/ml insulin (Sigma), 0.5 g/ml hydrocortisone (Sigma), and 20 ng/ml EGF (Cell Guidance Systems)
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How to use: The lotion is used on wet skin with gentle massage, then rinsed thoroughly
The labeled use period is calibrated to the labeled composition
Material supplied here is the full 43-residue sequence, evidenced by a mass spectrometry result of 4964.4 for the singly protonated ion on the published certificate of analysis
