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At ~50% confluency, cells were co-transfected with full-length Flag-tagged or MYC-tagged substrate constructs and HA-tagged protease constructs using Lipofectamine 3000

April 19, 2024

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Then literatures reported the Ala177Val mutation has no influence on the kinetic parameters of bacterial MTHFR, but rather reduces enzyme stability and affinity for cofactor, and thus increases the tendency to form inactive enzyme via flavin dissociation, compared to the wild-type enzyme 27