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All the above operations were performed according to the manufacturers instructions

The experiment was divided into a normal control group, a radiotherapy model group, and a radiotherapy+IGF-1 LR3 treatment group (1mg/kg, orally taken from the first day after radiotherapy, once a day, for 7 consecutive days)

On the contrary, glutathione helps detoxify the liver, which is one of the most important organs for processing fats and eliminating toxins from the body

The assay buffer consisted of DMEM without phenol red (Gibco #11880-028) supplemented with 10 mM HEPES (Gibco #15630-056), 1X GlutaMAX (Gibco #35020-038), and 1% (w/v) ovalbumin (Sigma A5503), 0.1% (v/v) Pluronic F-68 (Gibco, #24040-032)

That is why it is typically given as a daily injection, often at night to mirror natural growth-hormone rhythm

The role of glutathione S-transferases (GSTs) in insecticide resistance in crop pests and disease vectors