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The following formulas were used to determine the numerical density (NV), the total number of hepatocyte nuclei, and the Kupffer cells 46,47 : where Q describes the number of the cells counted, A describes the total areas of unbiased counting frames (the area of each frame is 1205.5 m 2 ), h is the height of the dissector (15 m), t is the mean sections thickness of all fields assessed by a microcator (~ 22 m), and BA (Block advance) is the sections thickness of the microtome set at 25 m