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van den Broek-Altenburg E, et al

Ca 2+ is a master regulator of eryptosis, mediating the Gardos effect-associated RBC dehydration and reduction of cell volume (cell shrinkage), calpain activation-mediated cytoskeleton remodeling (membrane blebbing), and PS externalization, which are major hallmarks of eryptosis

The healthy gut microbiome maintains systemic homeostasis by regulating bile acid and short-chain fatty acid (SCFA) metabolism, with SCFAs acting as key anti-inflammatory agents [55,56,57]

All efforts were made to minimize animal suffering and to reduce the number of animals used

However, this overcompensation leads to cellular lipid deposition, accumulation of metabolites, and activation of metabolic bypass pathways