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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

M., Hirst, W

2010 Jul-Sep;9(3):263-8 Pedrazini Mc, Arajo Vc, Montalli Va

Ethical Approval and Consent to Participate The melanotic schwannoma and control tissues were collected with the approval of the Ethics Committee of Air Force Military Medical University (Approval number: KY20232085-D-3, Date: 2023-03-07) and written informed consent was obtained from the specimen donors or their families

The MT-II scaffold has since generated a large derivative family through substitutions at positions 6, 7 and 9, including N-methylated variants used to obtain MC1R selectivity