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Brain endothelial cells are constitutively glycolytic, and pericyte function depends partly on endothelial metabolic support [14, 17, 18]

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Peptides were eluted over 60 min using a binary buffer system consisting of buffer A (0.1% formic acid) and buffer B (80% ACN, 0.1% formic acid)

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Hb signal intensity within TH-positive neuron surfaces was quantified, and colocalization analysis was performed to evaluate Hb expression in both GFAP-positive astrocytes and TH-positive neurons