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Further processing, including cell segmentation, was performed in MATLAB software (MathWorks), focusing on measuring intensity-weighted fluorescence lifetimes () and calculating the free/bound NAD(P)H ratio [44]

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coli DH10Bac strain (Biomed, BC112-01) to construct the recombinant bacmids

Methionine is an essential amino acid, and selenium is an antioxidant that acts as a cofactor for glutathione peroxidase which neutralizes hydrogen peroxide (see Figure 1)

Plates were then centrifuged at 800 g for 30 min, and the supernatant transferred into new plates