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This higher activity results from the insensitivity of ETC to FCCP treatment and is consistent with the limited reserve capacity of Gclc -deficient FoB (Fig

The same study also detected that more proinflammatory molecules, such as interferon and interleukin-1 were produced in the selenium-deficient group because multiple genes involved their upstream pathways, including UPS18 , DDX58 , cGAS , and TLR3 were upregulated, which further supported the conclusion that selenium deficiency could resulted in mitochondrial DNA damage and disorder energy production of cardiac cells (169)

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Blocking was performed with PBS 1 0.5% BSA 0.1% saponin (w/v/v), between incubations, cells were rinsed three times with PBS 1, for 5 min

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