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After washing with TBS containing 0.1% Tween 20, the membranes were incubated for 30 min at 25 C with TBS containing 0.1% Tween 20, 1% BSA, and horseradish peroxidase-conjugated anti-mouse (CST, 7076) or anti-rabbit (CST, 7074) secondary antibodies before visualization using Western Lightning ECL Pro (Perkin Elmer, NEL120001EA)

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The observed LC-MS m/z will depend on the charge state, adducts, and data-processing conventions

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These effects result from the peptide's ability to stimulate dermal papilla cells, the specialized fibroblasts that control hair follicle cycling and growth