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Briefly, proteins were subjected to sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-10% PAGE) under reducing conditions in Laemmlis buffer system followed by transfer to nitrocellulose membranes 19

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Effects on soluble sugar, proline and oxidative stress markers (MDA and H 2 O 2 ) Soluble sugars not only serve as energy sources but also act as osmotic regulators, stabilizing cell membranes and protoplasts 71

2014;27(277C):613

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