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Then, the MB was added at 5-serial dilution concentrations (1.25 to 20 M) and incubated for 24, 48, or 72 h (37 C and 5% CO 2 )

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5.4 Trace elements The few trace elements Cu, Mn, Zn, Fe, and Se, which are essential to the bodys antioxidant system are discussed in this section

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