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Next, the supernatant was collected after centrifuging at 1000 rpm for 10 min at 4 C, and then measured at 532 nm with a microplate reader

We thus designed RT (Fig

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Its protonated epsilon-amino group carries positive charge that affects solubility, chromatographic retention and interaction with polyanionic matrix components, and in a physiological setting the lysine face is implicated in ternary complex formation with serum albumin, which has its own high-affinity N-terminal copper site